Jean-Luc Da Lage

Member of the Genome Team
Researcher CNRS
PhD student Paris VII University  (1990), HDR (2003)

1397745702_aiga_telephone +33 (0)1 69 15 49 72
1397745633_aiga_mail Jean-Luc.Da-Lage[@]

Research topics

Molecular evolution of alpha-amylase genes and proteins in Metazoa. Functional evolution and adaptation; gene duplications and multigene families; intron evolution; horizontal or lateral gene transfer.

Alpha-amylase is a major enzyme of nutrition metabolism. It hydrolyses starch and other carbohydrates such as glycogene in smaller sugars (maltose). Amylase is present throughout the living world, Bacteria, Archae, Plants, Fungi, Animals.

In many organisms, it has been shown that the coding gene for alpha-amylase, Amy, exists in several copies. The duplications may have occurred independently even in closely related lineages. We aim at understanding the evolutionary significance of this tendency to get multiple Amy genes, with regard to the function of the enzyme, and the gene divergences in sequence and expression patterns.

Some species are suitable models in this study. This is the case of Drosophila ananassae from which seven genes were cloned, more or less related to each others. They were localized on several chromosomal arms and show different expression patterns. In addition, the intron-exon structure may be different not only between species but also among the copies of a single species. This lead us to investigate more carefully the evolutionary history of the Amy gene structure in Animals, and more generally use it as a case study of the dynamics of introns.

We are also interested in the evolution of the alpha-amylase protein itself. We have sought recurrent natural variations in the animal kingdom, which may be indicative of adaptation at the molecular level, and what do these adaptations consist in. After detection of several such motifs of interest, we aim at studying more functionally the observed variations by enzymatic engineering, by in vitro production of a control amylase, and several mutants harboring the recurrent variations observed in nature.


iconePdf FELLER G., BONNEAU M. and DA LAGE, J.-L. (2021). Amyrel, a novel glucose-forming α-amylase from Drosophila with 4-α-glucanotransferase activity by disproportionation and hydrolysis of maltooligosaccharides. Glycobiology. 31 (9):1134-1144.

iconePdf MULLER H., OGEREAU D., DA LAGE J.-L., CAPDEVIELLE C., POLLET N., FORTUNA T., JEANNETTE R., KAISER L. and GILBERT C. (2021). Draft genome of the Mediterranean corn borer, Sesamia nonagrioides, a major pest of maize. Genes, Genomes, Genetics. 11(7):jkab155. doi: 10.1093/g3journal/jkab155.

BICHANG’A G. B., DA LAGE J.-L., SAMBAI K., MULE S., LE RU B., KAISER L., JUMA G., MAINA, E. N. and CALATAYUD P.-A. (2020). Salivary α-amylase of stem borer hosts determines host recognition and acceptance for oviposition by Cotesia spp. (Hymenoptera, Braconidae). In : Calatayud, P.-A., Clarke, C. W., Sforza, R. F. H., Ndemah, R. N., Nyamukondiwa, C., eds. (2020). Parasitoids’ Ecology and Evolution. Lausanne: Frontiers Media SA. DOI: 10.3389/978-2-88963-480-4

iconePdfDESIDERATO A., BARBEITOS M., GILBERT C. and DA LAGE J.-L. (2020). Horizontal transfer and gene loss shaped the evolution of alpha-amylases in bilaterians. Genes, Genomes, Genetics. 10(2):709-719.

DA LAGE J.-L., THOMAS G. W. C., BONNEAU M. and COURTIER-ORGOGOZO V. (2019). Evolution of salivary glue genes in Drosophila species. BMC Evol. Biol. 19 :36. DOI: 10.1186/s12862-019-1364-9

iconePdfBICHANG’A G., DA LAGE J.-L., SAMBAI K., MULE S., LE RU B., KAISER-ARNAULD L., JUMA G., MAINA E. N. and CALATAYUD P.-A. (2018). Salivary α-amylase of stem borer hosts determines host recognition and acceptance for oviposition by Cotesia spp. (Hymenoptera, Braconidae). Front. Ecol. Evol. 6 : DOI: 10.3389/fevo.2018.00228

iconePdfDA LAGE J-L. (2018) The amylases of insects. Int. J. Insect Sci. 10 :1-14.

iconePdfBICHANG’A G., DA LAGE J-L., CAPDEVIELLE-DULAC C., ZIVY M., BALLIAU T., SAMBAI K., LE RU B., KAISER-ARNAULD L., JUMA G., NJOKI MWANGI MAINA E., and CALATAYUD P.-A. (2018) α-Amylase Mediates Host Acceptance in the Braconid Parasitoid Cotesia flavipes. J. Chem Ecol. 44 (11) :1030-1039.

iconePdfDA LAGE J-L. (2017) An optional C-terminal domain is ancestral in α-amylases of bilaterian animals. Amylase 1:24-36

iconePdf CLAISSE G., FELLER G., BONNEAU M., DA LAGE J-L. (2016) A single amino-acid substitution toggles chloride dependence of the alpha-amylase paralog amyrel in Drosophila melanogaster and Drosophila virilis species. Insect Biochem. Mol. Biol. 75:70-77

iconePdf COMMIN C., AUMONT-NICAISE M., CLAISSE G., FELLER G., DA LAGE J-L. (2013) Enzymatic characterization of recombinant α-amylase in the Drosophila melanogaster species subgroup: is there an effect of specialization on digestive enzyme? Genes Genet. Sys. 88:251-259

iconePdf DA LAGE J.-L., BINDER M., HUA VAN A., JANECEK S., CASANE D. (2013) Gene make-up: rapid and massive intron gains after horizontal transfer of a bacterial alpha-amylase gene to Basidiomycetes. BMC Evol. Biol. 13: 40

iconePdf CHAUMOT A., DA LAGE J-L., MAESTRO O., MARTIN D., IWEMA T., BRUNET F., BELLES X., LAUDET V., BONNETON F. ( 2012) Molecular adaptation and resilience of the insect’s nuclear receptor USP. BMC Evol. Biol. 12: 199

iconePdf CIPOLLA A., DELBRASSINE F., DA LAGE J-L., FELLER G. (2012) Temperature adaptation in psychrophilic, mesophilic and thermophilic chloride-dependent alpha-amylases. Biochimie 94(9): 1943-1950

iconePdfFELLER G., DEHARENG D., DA LAGE J-L. (2011) How to remain non-folded and pliable: the linkers in modular alpha-amylases as a case study. FEBS J. 278: 2333

iconePdfDA LAGE J-L., MACZKOWIAK F. , CARIOU M-L. (2011) Phylogenetic distribution of intron positions in alpha-amylase genes of Bilateria suggests numerous gains and losses. PLoS ONE 6(5): e19673

iconePdfYASSIN A.,DA LAGE J.L., DAVID J.R., KONDO M., MADI-RAVAZZI L., PRIGENT S., TODA M.J. (2010) Polyphyly of the Zaprionus Genus Group (Diptera: Drosophilidae). Molecular Phylogenetics and Evolution 55: 335

iconePdfYASSIN A., AMABIS J.M., DA LAGE J-L., DEBIAIS-THIBAUD M., DAVID J.R. (2010). On the relationship between Zaprionus spinipilus Chassagnard & McEvey and Z. vittiger Coquillett, the type species of the genus Zaprionus (Diptera: Drosophilidae). Ann. Soc. Entomol. Fr. 46 (3-4)

iconePdfMCEVEY S. F., SCHIFFER M., DA LAGE J-L., DAVID J. R., LEMEUNIER F., JOLY D., CAPY P. , CARIOU M-L. (2008) Comments on the proposed conservation of usage of Drosophila Fallén, 1823 (Insecta, Diptera). Bulletin of Zoological Nomenclature 65(2): 147

iconePdfLEGENDRE A., MIAO X.X., DA LAGE J.-L., WICKER-THOMAS, C. (2008) Evolution of a desaturase involved in female pheromonal cuticular hydrocarbon biosynthesis and courtship behavior in Drosophila. Insect Biochem Mol. Biol. 38(2): 244

iconePdf SCHäRER L., DA LAGE J.-L., JOLY D. (2008) Evolution of testicular architecture in the Drosophilidae: a role for sperm length. BMC Evol. Biol. 8: 143

iconePdf YASSIN, A., ARARIPE, L.O., CAPY, P., DA LAGE, J-L., KLACZKO L.B., MAISONHAUTE C., OGEREAU D., DAVID J.R. (2008) Grafting the molecular phylogenetic tree with morphological branches to reconstruct the evolutionary history of the genus Zaprionus (Diptera : Drosophilidae). Molecular Phylogenetics and Evolution 47: 903

iconePdfDA LAGE J-L, DANCHIN E.G.J., CASANE D. (2007) Where do animal alpha-amylases come from? An interkingdom trip. FEBS Letters 581(21): 3927-3935

iconePdfDA LAGE, J-L., KERGOAT, G. J., MACZKOWIAK, F., SILVAIN, J-F., CARIOU, M-L. and LACHAISE, D. (2007) A phylogeny of Drosophilidae using the Amyrel gene: questioning the Drosophila melanogaster species group boundaries. Journal of Zoological Systematics and Evolutionary Research 45(1): 47

iconePdf PREAT, T., DA LAGE, J-L., COLLEAUX, L., DIDELOT, G., MOLINARI, F., TCHENIO, P., MILHIET, E., MUNNICH, A., AND CARIOU, M-L. (2007) Response to comment on “Tequila, a neurotrypsin ortholog, regulates long-term memory formation in Drosophila. Science 316: 1698

iconePdfMACZKOWIAK, F.,DA LAGE, J-L. (2006) Origin and evolution of the Amyrel gene in the alpha-amylase family of Diptera. Genetica 128: 145

iconePdfDA LAGE J-L., FELLER G., JANECEK S. (2004) Horizontal gene transfer from Eukarya to Bacteria: the alpha-amylase model. Cell. Mol. Life Sci. 61(1): 97

iconePdfDA LAGE, J-L, MAISONHAUTE, C., MACZKOWIAK, F., CARIOU, M-L. (2003) A nested alpha-amylase gene in Drosophila ananassae. J. Mol. Evol. 57(3): 355

iconePdf ZHANG Z., INOMATA N., CARIOU M-L, DA LAGE J-L., YAMAZAKI, T. (2003) Phylogeny and evolution of the amylase multigenes in Drosophila montium species subgroup. J. Mol. Evol. 56: 121

iconePdfDA LAGE J-L, VAN WORMHOUDT A., CARIOU M-L (2002) Diversity and evolution of the alpha-amylase genes in animals. Biologia, Bratislava 57: 181

iconePdf CARIOU ML., SILVAIN J-F., DAUBIN V., DA LAGE J-L., LACHAISE D. (2001) Divergence between Drosophila santomea and allopatric or sympatric populations of D. yakuba using paralogous amylase genes and migration scenarios along the Cameroon volcanic line. Mol. Ecol. 10: 649

iconePdfDA LAGE, J.-L., MACZKOWIAK, F., CARIOU, M.-L. (2000) Molecular characterization and evolution of the amylase multigene family of Drosophila ananassae. J. Mol. Evol 51: 391

iconePdfRICHARDS G., DA LAGE J-L., HUET F. and RUIZ C. (1999) The acquisition of competence to respond to ecdysone in Drosophila is transcript specific. Mech. Dev. 82(82): 131

iconePdfDA LAGE J-L, RENARD E, CHARTOIS F, LEMEUNIER F, CARIOU M-L (1998) Amyrel, a paralogous gene of the amylase gene family in Drosophila melanogaster and the Sophophora subgenus. Proc. Natl. Acad. Sci. USA 95: 6848

iconePdfDA LAGE J-L., KLARENBERG A., CARIOU M-L. (1996) Sex-, stage- and tissue-specific expression of amylase genes in Drosophila ananassae. Heredity 76: 9

iconePdfDA LAGE J-L., WEGNEZ M., CARIOU M-L.(1996) Distribution and evolution of introns in Drosophila amylase genes. J. Mol. Evol. 43: 334

iconePdfHUET F., DA LAGE J-L., RUIZ C., RICHARDS G. (1996) The role of ecdysone in the induction and maintenance of hsp27 transcripts during larval and prepupal development of Drosophila. Dev. Genes Evol. 206: 326

CARIOU M-L., DA LAGE J-L. (1993) Isozyme polymorphisme. In: Drosophila ananassae, genetical and biological aspects. YN Tobari, Ed., Japan Scientific Societies Press, Karger, Tokyo 0: 160

DA LAGE J-L., CARIOU M-L. (1993) Organisation and structure of the amylase gene cluster. In: Drosophila ananassae, genetical and biological aspects. YN Tobari, Ed., Japan Scientific Societies Press, Karger, Tokyo 0: 171

iconePdfDA LAGE J-L., LEMEUNIER F., CARIOU M-L., DAVID J.R. (1992) Multiple amylase genes in Drosophila ananassae and related species. Genet. Res. Camb. 59: 85

iconePdfDA LAGE J-L, CAPY, P., DAVID, J.R. (1990) Starvation and desiccation tolerance in Drosophila melanogaster: differences between European, North African and Afrotropical populations. Genet. Sel. Evol. 22: 381

iconePdfDA LAGE J-L., CAPY P., DAVID J.R. (1989) Starvation and desiccation tolerance in Drosophila melanogaster adults: effects of environmental temperature. J. Insect Physiol. 35(6): 453

iconePdfDA LAGE J-L., CARIOU M-L., DAVID J.R. (1989) Geographical polymorphism of amylase in Drosophila ananassae and its relatives. Heredity 63: 67

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