M1 or M2 project<\/p>\n
Les alpha-amylases forment une famille multig\u00e9nique chez de nombreux organismes. Cette tendance \u00e0 avoir des copies
\nmultiples, survenue ind\u00e9pendamment dans de nombreuses esp\u00e8ces, sugg\u00e8re un int\u00e9r\u00eat adaptatif: Mais quelles sont les
\ndiff\u00e9rences fonctionnelles entre les diff\u00e9rentes copies? Chez les Dipt\u00e8res muscomorphes (les mouches vraies) existe un
\nparalogue appel\u00e9 Amyrel. Tr\u00e8s divergent des amylases classiques en s\u00e9quence, sa fonction reste \u00e9nigmatique. On se propose
\nd’\u00e9tudier sa r\u00e9gulation par la dissection du promoteur. Des drosophiles ont \u00e9t\u00e9 transform\u00e9es par des constructions exprimant la
\nGFP sous le contr\u00f4le du promoteur Amyrel entier ou plus ou moins d\u00e9l\u00e9t\u00e9. Elles serviront de base aux \u00e9tudes de r\u00e9gulation
\ntissulaire, temporelle, et par l’alimentation (r\u00e9pression \u00e9ventuelle par les sucres). De nouvelles constructions seront entreprises.
\nLe travail fera appel \u00e0 des techniques de biologie mol\u00e9culaire telles que la qRT-PCR, des dissections de larves et d’adultes,
\nl’observation sous macroscope et microscope en \u00e9pifluorescence. L’enzymologie d’Amyrel, qui est une enzyme active, sera pr\u00e9cis\u00e9e.<\/p>\n
Contact: Jean-Luc Da Lage (jean-luc.da-lage@universite-paris-saclay.fr)<\/p>\n","protected":false},"excerpt":{"rendered":"
M1 or M2 project Les alpha-amylases forment une famille multig\u00e9nique chez de nombreux organismes. Cette tendance \u00e0 avoir des copies multiples, survenue ind\u00e9pendamment dans de nombreuses esp\u00e8ces, sugg\u00e8re un int\u00e9r\u00eat adaptatif: Mais quelles sont les diff\u00e9rences fonctionnelles entre les diff\u00e9rentes copies? Chez les Dipt\u00e8res muscomorphes (les mouches vraies) existe un paralogue appel\u00e9 Amyrel. Tr\u00e8s divergent […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_themeisle_gutenberg_block_has_review":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1],"tags":[],"class_list":["post-7872","post","type-post","status-publish","format-standard","hentry","category-non-classe"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/7872","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7872"}],"version-history":[{"count":0,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/7872\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7872"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7872"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7872"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}