{"id":234,"date":"2013-11-22T14:44:34","date_gmt":"2013-11-22T13:44:34","guid":{"rendered":"https:\/\/www.egce.universite-paris-saclay.fr\/?p=234"},"modified":"2013-11-22T14:44:34","modified_gmt":"2013-11-22T13:44:34","slug":"pheromones-et-ponte-chez-la-drosophile","status":"publish","type":"post","link":"https:\/\/www.egce.universite-paris-saclay.fr\/?p=234","title":{"rendered":"Ph\u00e9romones et ponte chez la Drosophile"},"content":{"rendered":"

Nom de l’\u00e9quipe d’accueil<\/strong>
\nG\u00e9n\u00e9tique \u00c9volutive, Reproduction et Adaptation des drosophiles<\/p>\n

Responsable<\/strong>
\nWicker-Thomas<\/p>\n

Profil du candidat<\/strong>
\nInt\u00e9ress\u00e9(e) par la g\u00e9n\u00e9tique, le comportement, la biologie mol\u00e9culaire<\/p>\n

Descriptif du stage<\/strong>
\nChez la Drosophile, des ph\u00e9romones sexuelles situ\u00e9es sur la cuticule ont un r\u00f4le tr\u00e8s important dans le comportement de cour qui pr\u00e9c\u00e8de l\u2019accouplement. Ces ph\u00e9romones sont des hydrocarbures \u00e0 tr\u00e8s longue cha\u00eene, synth\u00e9tis\u00e9s \u00e0 partir d\u2019acides gras dans des cellules \u00e9pidermiques internes, les oenocytes. Elles sont ensuite export\u00e9es sur la cuticule par un m\u00e9canisme encore inconnu. L\u2019accouplement entra\u00eene une baisse de la synth\u00e8se des ph\u00e9romones cuticulaires et une augmentation de la ponte. Nous venons de mettre en \u00e9vidence l\u2019existence d\u2019un compos\u00e9 lipidique synth\u00e9tis\u00e9 par les oenocytes et \u00e0 destination des ovaires indispensable \u00e0 la ponte. L\u2019hypoth\u00e8se est qu\u2019une partie de la synth\u00e8se des acides gras pr\u00e9curseurs des ph\u00e9romones pourrait \u00eatre d\u00e9tourn\u00e9e au profit des ovaires, d\u00e9clenchant la ponte. Nous proposons d\u2019utiliser le syst\u00e8me d\u2019inactivation de g\u00e8nes par RNA interf\u00e9rence (RNAi) pour \u00e9tudier : – les g\u00e8nes de biosynth\u00e8se des acides gras dans les oenocytes qui d\u00e9clenchent la ponte – le transport des ph\u00e9romones des oenocytes vers la cuticule – le transport des lipides des oenocytes vers les ovaires. Cette \u00e9tude se fera gr\u00e2ce \u00e0 l\u2019utilisation de m\u00e9thodes biochimiques (analyse des ph\u00e9romones par chromatographie en phase gazeuse) et g\u00e9n\u00e9tiques (lign\u00e9es GAL4 ; mutants UAS-RNAi). Nous avons d\u00e9j\u00e0 s\u00e9lectionn\u00e9 quelques g\u00e8nes candidats et si les r\u00e9sultats le permettent, une \u00e9tude d\u2019expression des g\u00e8nes cible avant et apr\u00e8s accouplement par q-RT-PCR pourrait \u00eatre commenc\u00e9e.<\/p>\n

R\u00e9f\u00e9rences<\/strong>
\nR\u00e9f\u00e9rences Chertemps T., Duportets L., Labeur C., Ueyama M., Wicker-Thomas C. 2006 A female specific desaturase gene responsible for diene hydrocarbon biosynthesis and courtship behavior in D. melanogaster. Insect Mol. Biol. 15, 465-473. Chertemps T., Duportets L., Labeur C., Ueda R., Takahashi K., Saigo K., Wicker-Thomas C. 2007 A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA.104, 4273-4278<\/p>\n

Mots clefs<\/strong><\/p>\n

Contact<\/strong>
\nWicker-Thomas Claude Email : claude.wicker-thomas@universite-paris-saclay.fr T\u00e9l : 01 69 82 37 08<\/p>\n

Date de validit\u00e9<\/strong>
\nMercredi 15 Janvier 2014<\/p>\n

Dur\u00e9e (mois)<\/strong>
\n0<\/p>\n

Date de publication du stage<\/strong>
\nVendredi 23 Ao\u00fbt 2013<\/p>\n","protected":false},"excerpt":{"rendered":"

Nom de l’\u00e9quipe d’accueil G\u00e9n\u00e9tique \u00c9volutive, Reproduction et Adaptation des drosophiles Responsable Wicker-Thomas Profil du candidat Int\u00e9ress\u00e9(e) par la g\u00e9n\u00e9tique, le comportement, la biologie mol\u00e9culaire Descriptif du stage Chez la Drosophile, des ph\u00e9romones sexuelles situ\u00e9es sur la cuticule ont un r\u00f4le tr\u00e8s important dans le comportement de cour qui pr\u00e9c\u00e8de l\u2019accouplement. Ces ph\u00e9romones sont des […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_themeisle_gutenberg_block_has_review":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[29,30],"tags":[],"class_list":["post-234","post","type-post","status-publish","format-standard","hentry","category-master-i","category-master-ii"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/234","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=234"}],"version-history":[{"count":0,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/234\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=234"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=234"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=234"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}