{"id":18984,"date":"2024-05-08T10:50:21","date_gmt":"2024-05-08T08:50:21","guid":{"rendered":"https:\/\/www.egce.universite-paris-saclay.fr\/?p=18984"},"modified":"2024-05-08T12:38:30","modified_gmt":"2024-05-08T10:38:30","slug":"methodologies-genomes-et-evolution","status":"publish","type":"post","link":"https:\/\/www.egce.universite-paris-saclay.fr\/?p=18984","title":{"rendered":"M\u00e9thodologies G\u00e9nomes et Evolution"},"content":{"rendered":"\n

M\u00e9thodologies G\u00e9nomes et Evolution<\/a><\/strong><\/h2>\n\n\n\n

Nous utilisons des approaches experimentale<\/strong>, bioinformatique<\/strong>, et th\u00e9oriques<\/strong>. Nos mod\u00e8les sont principalement des mod\u00e8les animaux<\/strong> comme les Arthropodes<\/strong> (Les dipt\u00e8res inclus les Drosophiles, les gu\u00eapes \u00e0 galles ou les parasito\u00efdes, les cloportes) et les Amphibiens<\/strong>. Il s’agit d’organismes mod\u00e8les \u00e9lev\u00e9s au laboratoire ou collect\u00e9s dans la nature. Pour \u00e9tudier les g\u00e9nomes et leur \u00e9volution, nous utilisons les technologies NGS combin\u00e9es \u00e0 de la transgen\u00e8se et des techniques de biologies mol\u00e9culaire, g\u00e9n\u00e9tique, cytologies, \u00e0 diff\u00e9rentes \u00e9chelles, de l’individu \u00e0 des groupes d’esp\u00e8ces.<\/strong> Notre approache th\u00e9orique utilise le cadre de la g\u00e9n\u00e9tique des populations <\/strong> et repose principalement sur des simulations.<\/p>\n\n\n\n

Travaux exp\u00e9rimentaux<\/strong><\/h3>\n\n\n\n
mat\u00e9riels vivants<\/mark><\/strong><\/h6>\n\n\n\n

Nos specimens proviennent soit de la nature, collect\u00e9s au cours de missions de terrain, ou sont des populations de laboratoire, maintenu dans notre insectarium ou notre aquarium vert\u00e9br\u00e9s<\/p>\n\n\n\n

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We collect insects and amphibians in France, French Guiana, Costa Rica, Gabon, Cameroon….<\/p>\n<\/div>\n\n\n\n

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Drosophila rearing<\/p>\n<\/div>\n<\/div><\/div>\n\n\n\n

Transgen\u00e8se<\/mark><\/strong><\/h6>\n\n\n\n

Certains de nos projets implique de manipuler le g\u00e9nome. Nous utilisons notre plateau de transgen\u00e8se pour produire des lign\u00e9es de drosophiles g\u00e9n\u00e9tiquement modifi\u00e9es (Drosophila melanogaster<\/em> et autres esp\u00e8ces de drosophile).<\/p>\n\n\n\n

techniques mol\u00e9culair<\/mark><\/strong>e<\/strong>s<\/mark><\/h6>\n\n\n\n

Biologie mol\u00e9culaire, cytologie, biochimie…<\/p>\n\n\n\n

G\u00e9nomique et transcriptomique (NGS)<\/strong><\/h3>\n\n\n\n

Une grande partie de nos donn\u00e9es correspondent \u00e0 des donn\u00e9es de s\u00e9quen\u00e7age (G\u00e9nomes assembl\u00e9s, sequen\u00e7age a courtes ou longues lectures, S\u00e9que\u00e7age ARN et petits ARN). Certaines donn\u00e9es sont produites au laboratoire, gr\u00e2ce \u00e0 notre plateau de s\u00e9quen\u00e7age (Sanger, MinION). Nous utilisons ces donner pour r\u00e9aliser des analyses g\u00e9nomiques compar\u00e9es, des phylogenies, des analyse d’\u00e9volution mol\u00e9culaires. Pour cela nous sommes amen\u00e9s \u00e0 developper nos propres pipelines<\/em> bioinformatiques.<\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

M\u00e9thodologies G\u00e9nomes et Evolution Nous utilisons des approaches experimentale, bioinformatique, et th\u00e9oriques. Nos mod\u00e8les sont principalement des mod\u00e8les animaux comme les Arthropodes (Les dipt\u00e8res inclus les Drosophiles, les gu\u00eapes \u00e0 galles ou les parasito\u00efdes, les cloportes) et les Amphibiens. Il s’agit d’organismes mod\u00e8les \u00e9lev\u00e9s au laboratoire ou collect\u00e9s dans la nature. Pour \u00e9tudier les g\u00e9nomes […]<\/p>\n","protected":false},"author":41,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_themeisle_gutenberg_block_has_review":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[504],"tags":[],"class_list":["post-18984","post","type-post","status-publish","format-standard","hentry","category-pole-genomes"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/18984","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/users\/41"}],"replies":[{"embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18984"}],"version-history":[{"count":7,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/18984\/revisions"}],"predecessor-version":[{"id":19009,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=\/wp\/v2\/posts\/18984\/revisions\/19009"}],"wp:attachment":[{"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18984"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=18984"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.egce.universite-paris-saclay.fr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=18984"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}